The Serpentine are a race of snake-like humanoids that once ruled Ninjago, but were banished to five separate tombs, to separate the five warring tribes. They inhabited the world of Ninjago, after being released by Lloyd Garmadon. They consist of five tribes. Each tribe is further divided into four classes: Generals, Warriors, Soldiers, and Scouts. An additional rank, Snake King, rules over all five tribes. They have currently made their home in the Stone Army's former tomb.
They were created by the first Spinjitzu Master. Pythor made himself king of the Serpentine in the first season. Lord Garmadon was the Serpentine King in Season 2-Part 1. Skales is the current King of the Serpentine.
- “Had you released the Constrictai, the Venomari, or heaven forbid the Anacondrai, then we would have had a tussle.”
- ―Skales to Lloyd Garmadon
The Anacondrai were the most feared of all tribes. Since the Anacondrai were locked away without food, they became cannibalistic until Pythor was the last one remaining. They resemble Anacondas. Pythor is known to have the power to turn invisible. This is the only tribe that does not have a silver Fang Blade. The only known rank in the tribe is as follows:
- Pythor - The General of the Anacondrai.
The Constrictai are led by Skalidor. They are strong and large. Constrictai are capable of traveling under the ground, but can be located by following the raising of the ground as they tunnel under it. According to LEGO.com their staff's anti-venom allows you to breathe again after being constricted or choked by a Constrictai. They are orange and black in color. With the exception of Skalidor who has a snake tail, all Constrictai members have short legs. They seem to resemble Boa Constrictors. The ranks in this tribe are as follow:
- Skalidor - The heavyweight General of the Constrictai
- Bytar - The warrior of the Constrictai
- Chokun - a soldier-class member of the Constrictai
- Snike - a Scout-class member of the Constrictai.
The Fangpyre, led by Fangtom, are red and white and have large fangs. Their fangs can turn inanimate objects into living snake-like creatures. Vehicles will turn into living snake-cars, mechs, or wrecking balls. Each vehicle takes on snake attributes such as a tail and eyes. They are also able to bite living creatures and turn them into snake people. The venom slowly mutates the cells. However the venom is useless if the victims heart rate rises. They seem to resemble Pit vipers. The ranks of the Fangpyre are as following:
- Fangtom - The two-headed general
- Fangdam - Fangtom's two-headed Warrior
- Fang-Suei - A Fangpyre soldier
- Snappa - A Fangpyre scout
The Hypnobrai has been known to have two generals. Their original general, Slithraa, was challenged by his second-in-command, Skales, who won, which resulted in the latter replacing the former as the new general with Slithraa being demoted to a warrior.
The Hypnobrai have a dark blue, yellow, and grey colour scheme. They resemble the Spectacled (Indian) Cobra and have the same Specactled pattern on their hood. Their eyes in fact are red with spirals giving them a "dazzling" look which, when they use , hypnotizes their victims. The Hypnobrai are a Serpentine tribe with the power to hypnotize and control anyone. The ranks are as follows:
- Skales - The current Snake King and General of the Hypnobrai; former Warrior
- Slithraa - The current Warrior of the Hypnobrai; former General
- Mezmo - A Hypnobrai soldier
- Rattla - A Hypnobrai scout
- Skales Jr. - snake prince
- Selma - Snake Queen
The Venomari are led by Acidicus and live in toxic bogs of acid that only they can survive in. They are green in colour and have the ability to spray venom at an enemy. This venom sabotages the victim's vision and makes them hallucinate, making the victim see everyone turn into what they fear most. They have four eyes, unless they lose one, like Lasha, have scars, and are red, lime green, and dark green. They resemble spitting cobras. The ranks of the Venomari are as follows:
- Acidicus - The Venomari General
- Lizaru - The Warrior of the Venomari
- Spitta - A Soldier of the Venomari
- Lasha - A three-eyed Venomari Scout
With the help of Skales, Pythor successfully united the five Serpentine tribes by challenging the other generals for their staffs. Skales and Pythor were both wearing earplugs, so when Skales gave Pythor the sacred flute Skales just pretended to be annoyed and threw down his staff. All the other snake generals were so annoyed by the flute that they all threw down their staffs also. After defeating them, he dubbed himself the Snake King. However, after Pythor's defeat when he was eaten by the Great Devourer, Skales attempted to become the Snake King. He failed to when all of the Serpentine abandoned him and the other generals to follow Lord Garmadon.
Lord Garmadon ruled the Serpentine for the first half of Season 2. He would hit them, yell at them, and blame them for his failures. This made the Serpentine angry, so Skales abandoned Lord Garmadon in the middle of the ocean, and finally made himself the Snake King.
Skales became the current Snake King. Soon, he planned to dig beneath the surface with the Serpentine to get revenge on the citizens of Ninjago for locking them underground. The Serpentine ran into the Stone Army tomb, but got trapped there for sometime until after the Overlord's defeat.
The generals are the leader of their respective tribes. Generals are easily distinguished from other Serpentine in that rather than legs, they have a tail. Like warriors and soldiers, they also have a less humanoid shaped head than scouts. In addition to the tails, the generals are also normally in possession of their tribe's respective Snake Staff which is able to act as anti-venom to a tribe's power. For example, the Fangpyre staff would be able to return a person who has been bitten by a Fangpyre and is turning into a snake person to their normal state.
Generals are able to be debunked by being defeated by a Serpentine of a lower class, as seen when the Hypnobrai warrior, Skales, defeated the Hypnobrai general, Slithraa. Skales grew a tail while Slithraa lost his. This is likely the same for other tribes.
The Serpentine Generals are as follows;
The warriors are the second-in-command of their tribe. They have the same shaped head as their tribe's generals, but normally have different markings. Rather than having tails like the generals, warriors have humanoid legs.
As Pythor was the sole living Anacondrai member, there are no known warriors from his tribe.
Warriors are as follows;
Below warriors, are soldiers. Soldiers have humanoid legs like warriors and snake-like heads, but unlike the warriors, they are of a different shape than their respective generals'. They also have very large fangs. The soldiers typically have different head molds then the general and warrior and seem to look like each other such as with Constrictai and Fangpyre as well as Hypnobrai and Venomari
Soldiers are as follows;
It appears that the soldiers are the judges of the slither pit, as Mezmo, the Hypnobrai soldier was the judge of the Hypnobrai slither pit, and in All of Nothing, Fang-Suei was the judge of the slither pit in the snakes' underground base, and he is the Fangpyre soldier.
Scouts are at the bottom of the Serpentine social ladder. They have humanoid legs like warriors and soldiers, but also humanoid heads with a snake-like hood.
Scouts are as follows;
Vipers are not actually part of the social classes, but they are part of the Serpentine armies. The vipers are small and can fit into a minifigures hand like a whip. The young Great Devourer resembled a Venomari Toxic Viper, except it had frills behind its head. Also, its bite can turn the purest of things evil. Vipers can grow up and become an adult Serpentine. 
Vipers are as follows:
- Golden Viper
- Sly Viper
- Fangpyre Viper 
- Hypno Viper
- Unnamed Transparent Blue Viper
- Toxic Viper
- Unnamed Transparent Purple Viper
- Unnamed Transparent Orange Viper
The Serpentine once ruled Ninjago. Had it not been for the people of Ninjago and their sacred flutes which controlled the Serpentine, the Devourer would have consumed the land. The Great Devourer was banished to the lost city of Ouroboros and the five serpentine tribes into five tombs.
Years later, Lloyd Garmadon, son of Lord Garmadon, discovered and freed the Hypnobrai to get revenge on his uncle and his uncle's students. Freeing the Hypnobrai led to the release of the four other tribes; Constrictai, Venomari, Fangpyre, and Anacondrai.
Pythor P. Chumsworth, the last living Anacondrai, declares himself destined to reawaken the Great Devourer. To do so, he defeated the four generals of the other tribes in combat using a stolen sacred flute, given to him by Skales when no one noticed, and took their Snake Staffs, declaring himself the snake king. All Pythor needs to do at this moment is to find the four fang-blades of the Hypnobrai, Fanypyre, Venomari, and Constrictai tribes.
The Serpentine then found the four silver fang blades and released The Great Devourer. The Devourer ate Pythor and the rest of the Serpentine then realized that it was a bad idea to release it. They quickly go to the Fangpyre tomb and lock themselves inside. Fangtom blames the mishap on Skales, because he was Pythor's right hand man. However, the last we see of the serpentine is when the generals hear the Great Devourer has been destroyed and Skales says, "Someone's going to have to lead now." implying that he is going to take charge.
However, his first order resulted the Serpentine to be trapped underground again by the Stone Army.
In Rebooted, a mysterious hooded figure steals the Digital Overlord's hard drive. He turns Sensei Wu into Tech Wu. Him and Wu attack the Ninja at Ed and Edna's junkyard, after repowering the Nindroids using Electro Cobrai. The stranger leaves behind a white scale, which leads the Ninja to the idea that it is one of the Serpentine.
The Ninja go back to New Ninjago City and head underground, where they confront Skales. Skales, now married and with a child (Skales Jr.), tells the Ninja they are mistaken and they have converted to peace. Acidicus tells the Ninja of one of their legends, about how the Golden Master (Overlord) will end Ninjago as they know it. The Nindroids attack, and the Ninja discover that their power source is Electro Cobrai. As the Ninja head off, Skales announces that the only Serpentine known to be so fond of Electro Cobrai is...
Lloyd and Garmadon are traveling through the maze and are attacked by the Nindroids. They flee and are followed. Tech Wu, riding the Mechdragon, blocks their path, trapping Lloyd and his father. The hooded stranger comes behind Lloyd and reveals himself to be Pythor, now bleached white with purple markings. He tells Lloyd that Wu was lucky and escaped unharmed, but Pythor had digested in the Devourer's stomach for a long time. Wu pushes Garmadon off a cliff, making him fall into the ocean, and Pythor captures Lloyd.
- Hypnobrai - They were kept in an icy terrain styled cave with ice features.
- Fangpyre - They were kept in a graveyard near a mutated tree with two snake-like heads. It is misty and ghostly-looking.
- Constrictai - They were left in the desert in the Mountain of a Million Steps. They were in a dark cave inside the mountain with art and stories drawn on the wall.
- Venomari - These snakes were thankfully in their home the Toxic Bogs. They were kept inside a tree.
- Anacondrai - They were left in a horn shaped rock prison where they starved or got eaten except for Pythor. It had rock walls and was littered with the remains of Anacondrai.
9449 Ultra Sonic Raider
9442 Jay's Storm Fighter
9455 Fangpyre Mech
9443 Rattle Copter
9445 Fangpyre Truck Ambush
9457 Fangpyre Wrecking Ball
853404 Ninjago Magnet Set: Sensei Wu, Fangpyre and Ninja Jay
- The Serpentine can live for a very long time. This is shown when it is revealed that Skales and Fangtom were friends before they were locked in the tombs.
- Despite there being an Anacondrai staff in the TV show, one doesn't appear in any sets.
- It seems that every Soldier of the tribe is also the commentator for The Slither Pit.
- The Fangpyre's name and transylvanian accents references vampires.
- This are the second antagonists who had reformed with the exception of Pythor, the first being was the Skullkin.